對于很多科研汪來說(shuo)，細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)是(shi)脆(cui)弱(ruo)又重(zhong)要(yao)(yao)的(de)(de)(de)寶(bao)(bao)貝之(zhi)一(yi)(yi)，承擔著重(zhong)要(yao)(yao)的(de)(de)(de)實驗(yan)數據希(xi)望。尤其是(shi)在凍(dong)(dong)存復蘇(su)的(de)(de)(de)時候，經常會(hui)(hui)因為一(yi)(yi)些小細(xi)(xi)(xi)(xi)節導致(zhi)問題。比如：沒(mei)有控制好細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)的(de)(de)(de)生(sheng)長密(mi)度(du)細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)的(de)(de)(de)密(mi)度(du)對細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)的(de)(de)(de)生(sheng)存質(zhi)量有著重(zhong)要(yao)(yao)的(de)(de)(de)影響(xiang)，畢竟(jing)它們(men)是(shi)一(yi)(yi)群又不能擠(ji)著了也(ye)不能孤獨的(de)(de)(de)嬌貴寶(bao)(bao)寶(bao)(bao)。密(mi)度(du)過高(gao)會(hui)(hui)讓細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)沒(mei)有足夠的(de)(de)(de)生(sheng)存空間，密(mi)度(du)過低會(hui)(hui)讓細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)無(wu)法互(hu)相連接健康生(sheng)長。建議大家在細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)接種前，一(yi)(yi)定要(yao)(yao)調整好適(shi)合細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)生(sheng)長的(de)(de)(de)濃度(du)，提高(gao)細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)的(de)(de)(de)存活率。溫度(du)控制不達(da)標慢凍(dong)(dong)快融是(shi)細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)凍(dong)(dong)存復蘇(su)的(de)(de)(de)*關鍵詞之(zhi)一(yi)(yi)存細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)時需現(xian)配凍(dong)(dong)存液(ye)(如購買市面(mian)上通用的(de)(de)(de)含(han)血(xue)清細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)凍(dong)(dong)存液(ye)。杭(hang)州長沙(sha)無(wu)血(xue)清細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)凍(dong)(dong)存液(ye)

無(wu)血清(qing)細(xi)胞(bao)(bao)凍(dong)存(cun)液(ye)(ye)，含多種(zhong)保(bao)(bao)護(hu)(hu)劑成(cheng)分。一些保(bao)(bao)護(hu)(hu)劑，易于(yu)(yu)穿透細(xi)胞(bao)(bao)，避免細(xi)胞(bao)(bao)內部水分子形(xing)成(cheng)冰(bing)晶損傷(shang)細(xi)胞(bao)(bao)，同時另一些保(bao)(bao)護(hu)(hu)劑不能穿透細(xi)胞(bao)(bao)，但可以在冰(bing)晶形(xing)成(cheng)之前，優先結合細(xi)胞(bao)(bao)外(wai)的水分子，降(jiang)低(di)細(xi)胞(bao)(bao)外(wai)溶液(ye)(ye)的電(dian)解質濃度，減(jian)少陽(yang)離子進(jin)入(ru)細(xi)胞(bao)(bao)的數量(liang)，為(wei)多種(zhong)保(bao)(bao)護(hu)(hu)劑組合，在細(xi)胞(bao)(bao)內外(wai)進(jin)行雙(shuang)重(zhong)保(bao)(bao)護(hu)(hu)，較(jiao)大提(ti)高了細(xi)胞(bao)(bao)復蘇存(cun)活(huo)率。無(wu)血清(qing)細(xi)胞(bao)(bao)凍(dong)存(cun)液(ye)(ye)不含牛血清(qing)，無(wu)動物源組份。2-8℃即(ji)可穩(wen)定保(bao)(bao)存(cun)，無(wu)需(xu)(xu)冷凍(dong)，即(ji)取(qu)即(ji)用。凍(dong)存(cun)細(xi)胞(bao)(bao)，無(wu)需(xu)(xu)程序降(jiang)溫。凍(dong)存(cun)細(xi)胞(bao)(bao)復蘇率在90%以上(shang)。重(zhong)慶正規(gui)無(wu)血清(qing)細(xi)胞(bao)(bao)凍(dong)存(cun)液(ye)(ye)廠家(jia)批發價無(wu)血清(qing)細(xi)胞(bao)(bao)凍(dong)存(cun)液(ye)(ye)使用方法：加入(ru)適(shi)量(liang)的無(wu)血清(qing)型細(xi)胞(bao)(bao)凍(dong)存(cun)液(ye)(ye)于(yu)(yu)離心管中(zhong)。

無血(xue)清(qing)(qing)細(xi)(xi)(xi)胞(bao)(bao)凍(dong)(dong)存液(ye)(ye)的(de)(de)操作(zuo)規范(fan)：1、培養(yang)細(xi)(xi)(xi)胞(bao)(bao)至對數生(sheng)(sheng)長(chang)(chang)晚期，顯微鏡(jing)觀察其外觀、形態、有無污染等。選(xuan)擇狀態良好的(de)(de)細(xi)(xi)(xi)胞(bao)(bao)進行(xing)凍(dong)(dong)存操作(zuo)(注：貼壁生(sheng)(sheng)長(chang)(chang)細(xi)(xi)(xi)胞(bao)(bao)，用(yong)胰蛋白酶消(xiao)化并(bing)用(yong)完全(quan)培養(yang)基終止消(xiao)化，進行(xing)細(xi)(xi)(xi)胞(bao)(bao)計(ji)數;懸浮(fu)生(sheng)(sheng)長(chang)(chang)細(xi)(xi)(xi)胞(bao)(bao)，進行(xing)細(xi)(xi)(xi)胞(bao)(bao)計(ji)數)。2、500～1000g離(li)心5min，棄上(shang)清(qing)(qing)。3、加入適量的(de)(de)細(xi)(xi)(xi)胞(bao)(bao)凍(dong)(dong)存液(ye)(ye)，重懸細(xi)(xi)(xi)胞(bao)(bao)，使細(xi)(xi)(xi)胞(bao)(bao)濃(nong)度達到1～10×106/ml，置于凍(dong)(dong)存管中密(mi)閉(bi)。4、采取逐級(ji)降(jiang)溫保存：4℃放(fang)置20min，-20℃放(fang)置30min，-70℃過(guo)夜(ye)，置于液(ye)(ye)氮(dan)罐中長(chang)(chang)期存儲。注意：務(wu)必超凈(jing)工作(zuo)臺內無菌操作(zuo)，避免污染。雜交(jiao)瘤細(xi)(xi)(xi)胞(bao)(bao)凍(dong)(dong)存時(shi)應定期復蘇，以檢查細(xi)(xi)(xi)胞(bao)(bao)的(de)(de)活性(xing)和分(fen)泌抗體的(de)(de)穩定性(xing)。

無血清(qing)細(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)凍(dong)存(cun)(cun)液(ye)(ye)通用于(yu)各(ge)種動(dong)物細(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)株（tumour細(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)和(he)常規細(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)），大部(bu)分(fen)(fen)的(de)(de)干(gan)細(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)，凍(dong)存(cun)(cun)細(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)可在-80℃長期保(bao)(bao)存(cun)(cun)。不(bu)含動(dong)物源性(xing)(xing)蛋白(bai)，不(bu)含血清(qing)成(cheng)分(fen)(fen)，可有效減少各(ge)類(lei)細(xi)(xi)(xi)菌、病(bing)毒和(he)支(zhi)原體等污(wu)染(ran)，保(bao)(bao)證凍(dong)存(cun)(cun)細(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)的(de)(de)安(an)全。該凍(dong)存(cun)(cun)液(ye)(ye)含DMSO、葡萄糖(tang)等各(ge)種細(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)營養成(cheng)分(fen)(fen)，提高細(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)存(cun)(cun)活率(lv)和(he)活力，亦適合(he)于(yu)無血清(qing)培養細(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)和(he)蛋白(bai)表達細(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)。產(chan)品優勢(shi)：1、無需程序性(xing)(xing)降(jiang)溫(wen)，可直接(jie)將細(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)置于(yu)-80℃凍(dong)存(cun)(cun)，凍(dong)存(cun)(cun)液(ye)(ye)無需稀(xi)釋。2、細(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)可于(yu)-80℃長期保(bao)(bao)存(cun)(cun)。3、凍(dong)存(cun)(cun)液(ye)(ye)不(bu)含血清(qing)等，較大降(jiang)低細(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)污(wu)染(ran)的(de)(de)可能性(xing)(xing)。保(bao)(bao)存(cun)(cun)方(fang)法(fa)：冰(bing)袋運輸，4℃保(bao)(bao)存(cun)(cun)，保(bao)(bao)質期一年。無血清(qing)細(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)凍(dong)存(cun)(cun)液(ye)(ye)可用于(yu)其他類(lei)型哺乳動(dong)物細(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)的(de)(de)儲存(cun)(cun)。

無血(xue)(xue)清(qing)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)凍存(cun)(cun)液(ye)(ye)的(de)(de)使(shi)用(yong)(yong)(yong)(yong)方(fang)法及注意事項(xiang)：無血(xue)(xue)清(qing)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)凍存(cun)(cun)液(ye)(ye)：含多種保(bao)(bao)護(hu)(hu)(hu)(hu)劑(ji)成(cheng)分(fen)(fen),一些(xie)保(bao)(bao)護(hu)(hu)(hu)(hu)劑(ji),易(yi)于(yu)穿(chuan)透細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao),避免細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)內部水(shui)分(fen)(fen)子(zi)形成(cheng)冰(bing)晶(jing)損(sun)傷細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao),同時另一些(xie)保(bao)(bao)護(hu)(hu)(hu)(hu)劑(ji)不能穿(chuan)透細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao),但可(ke)以(yi)在冰(bing)晶(jing)形成(cheng)之前,優先(xian)結合細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)外的(de)(de)水(shui)分(fen)(fen)子(zi),降(jiang)低細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)外溶(rong)液(ye)(ye)的(de)(de)電解質濃(nong)度(du)(du),減少(shao)陽離子(zi)進入細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)的(de)(de)數(shu)量,我公司無血(xue)(xue)清(qing)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)凍存(cun)(cun)液(ye)(ye),為(wei)多種保(bao)(bao)護(hu)(hu)(hu)(hu)劑(ji)組合,在細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)內外進行雙(shuang)重(zhong)保(bao)(bao)護(hu)(hu)(hu)(hu),較大提(ti)高了(le)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)復蘇存(cun)(cun)活(huo)率。【產品用(yong)(yong)(yong)(yong)途】1.用(yong)(yong)(yong)(yong)于(yu)免疫(yi)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)及干細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)的(de)(de)存(cun)(cun)儲。2.細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)保(bao)(bao)藏中(zhong)(zhong)(zhong)心，用(yong)(yong)(yong)(yong)于(yu)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)株的(de)(de)長(chang)期保(bao)(bao)存(cun)(cun)。3.科研(yan)高校，細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)株凍存(cun)(cun)。4.醫院樣本庫細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)樣本保(bao)(bao)存(cun)(cun)。【使(shi)用(yong)(yong)(yong)(yong)方(fang)法】1、細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)凍存(cun)(cun)前準備（1）要(yao)求凍存(cun)(cun)的(de)(de)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)在對(dui)數(shu)生(sheng)長(chang)期，且活(huo)率大于(yu)90%。（2）計(ji)算細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)密度(du)(du)，一般要(yao)求密度(du)(du)在1-3x10cells/mL，不同細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)系請(qing)參(can)照附表(biao)。2、細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)凍存(cun)(cun)過程（1）將要(yao)凍存(cun)(cun)的(de)(de)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)懸液(ye)(ye)，進行細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)計(ji)數(shu)，計(ji)數(shu)后(hou)離心，8003min即(ji)可(ke)。度(du)(du)保(bao)(bao)存(cun)(cun)的(de)(de)無血(xue)(xue)清(qing)凍存(cun)(cun)液(ye)(ye)緩慢加入離心后(hou)的(de)(de)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)沉淀中(zhong)(zhong)(zhong)，根據密度(du)(du)調整細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)凍存(cun)(cun)液(ye)(ye)用(yong)(yong)(yong)(yong)量。（3）反復吹吸混勻后(hou)，分(fen)(fen)裝于(yu)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)凍存(cun)(cun)管(guan)中(zhong)(zhong)(zhong)，每管(guan)500ul-1000ul，（建議500ul/管(guan)）。無血(xue)(xue)清(qing)凍存(cun)(cun)液(ye)(ye)使(shi)用(yong)(yong)(yong)(yong)方(fang)法：將凍存(cun)(cun)管(guan)直接轉(zhuan)移(yi)至-80C水(shui)箱中(zhong)(zhong)(zhong)冷(leng)凍保(bao)(bao)存(cun)(cun)。廣州正(zheng)規無血(xue)(xue)清(qing)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)凍存(cun)(cun)液(ye)(ye)廠(chang)家直銷(xiao)

可(ke)以防(fang)止或減少冷(leng)凍冰晶對(dui)細(xi)胞的(de)損傷作用。杭州長沙無血清細(xi)胞凍存液(ye)

無(wu)(wu)血清快速細(xi)胞(bao)凍(dong)(dong)存(cun)(cun)(cun)(cun)液，通用(yong)(yong)(yong)于(yu)各(ge)(ge)種動(dong)(dong)物細(xi)胞(bao)株。特(te)別(bie)配(pei)方具有有效提高細(xi)胞(bao)凍(dong)(dong)存(cun)(cun)(cun)(cun)活(huo)率(lv)和(he)復蘇活(huo)力(li)。不(bu)含動(dong)(dong)物來源性(xing)蛋白，能(neng)減少各(ge)(ge)類(lei)病毒、霉菌和(he)支原(yuan)(yuan)體(ti)等(deng)的污染，確保(bao)凍(dong)(dong)存(cun)(cun)(cun)(cun)細(xi)胞(bao)安(an)全(quan)。既(ji)適用(yong)(yong)(yong)于(yu)一般培養細(xi)胞(bao)的凍(dong)(dong)存(cun)(cun)(cun)(cun)，也適用(yong)(yong)(yong)于(yu)無(wu)(wu)血清培養細(xi)胞(bao)和(he)蛋白表達細(xi)胞(bao)的凍(dong)(dong)存(cun)(cun)(cun)(cun)。產(chan)(chan)品(pin)特(te)色：高安(an)全(quan)性(xing)，完(wan)全(quan)使(shi)用(yong)(yong)(yong)醫藥品(pin)等(deng)級(ji)原(yuan)(yuan)料進(jin)行生(sheng)產(chan)(chan)，不(bu)含動(dong)(dong)物成分，病毒、霉菌和(he)支原(yuan)(yuan)體(ti)等(deng)污染可能(neng)性(xing)低，各(ge)(ge)批產(chan)(chan)品(pin)之間有更高的產(chan)(chan)品(pin)質(zhi)量一致性(xing)。細(xi)胞(bao)存(cun)(cun)(cun)(cun)活(huo)率(lv)高，無(wu)(wu)批次差異。完(wan)全(quan)凍(dong)(dong)存(cun)(cun)(cun)(cun)液配(pei)方，可直接(jie)使(shi)用(yong)(yong)(yong)，方便簡(jian)捷，可直接(jie)存(cun)(cun)(cun)(cun)放于(yu)-70℃冰箱凍(dong)(dong)存(cun)(cun)(cun)(cun)，無(wu)(wu)需經過(guo)費時(shi)(shi)的程序降溫過(guo)程（省(sheng)(sheng)時(shi)(shi)、省(sheng)(sheng)力(li)、省(sheng)(sheng)錢(qian)）。杭(hang)州長(chang)沙無(wu)(wu)血清細(xi)胞(bao)凍(dong)(dong)存(cun)(cun)(cun)(cun)液

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